Fashion a Fashion Pattern Snakeskin Boots Boots Boots Knee Size The Party Banquet Wedding Womens Thigh High Gift Spell Boots HETAO Martin Color Boots Large Over P87SF6Awwq
Fashion a Fashion Pattern Snakeskin Boots Boots Boots Knee Size The Party Banquet Wedding Womens Thigh High Gift Spell Boots HETAO Martin Color Boots Large Over P87SF6Awwq--minourossi.com
 
 
Fashion Womens Boots Fashion Wedding Color Boots a Martin Spell Size Pattern Party Boots Boots Large HETAO Knee Gift High Thigh The Snakeskin Over Boots Banquet

Fashion a Fashion Pattern Snakeskin Boots Boots Boots Knee Size The Party Banquet Wedding Womens Thigh High Gift Spell Boots HETAO Martin Color Boots Large Over P87SF6Awwq

Sigma offers over 200 modifications for both DNA and RNA oligonucleotides and probes. Sigma can attach fluorescent dyes to oligos at the 5'-end, 3'-end and internal positions. Our product offering includes common dye modifiers such as 6-FAM™, HEX™, and TET™, as well as exotic modifications.

In addition to standard fluorescent modifications, we have the capabilities to provide Coolred Printed Dress Women Lounge Tunic Tank Short Floral 5 Oversized PSP6nqarx including Molecular Beacons, LightCycler® Probes, and Scorpions® Probes.

Modification Categories

 

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Fluorescent Positions Excitation
Maximum (nm)
Emission
Maximum (nm)
Fluorescent Color
6-FAM™ (fluorescein) 5' End, 3' End 495 520 Green
ROX™ 5' End, 3' End 586 610 Orange
Cyanine 3 5' End, 3' End 549 566 Green-Yellow
Cyanine 5 5' End, 3' End 646 669 Red
Cyanine 5.5 5' End 675 694 Red
6-FAM dT 5' End, Internal 495 520 Green
HEX™ 5' End 535 556 Green
JOE™ 5' End, 3' End 529 555 Green
6-Carboxy-rhodamine 6G™ 5' End, 3' End 524 550 Green
TAMRA 3' End 557 583 Green-Yellow
TAMRA NHS Ester 5' End 557 583 Yellow
TET™ 5' End 521 536 Green
TxRd (Sulforhodamine 101-X) 5' End, 3' End 597 616 Red-Orange
A488 (Sulfonated Fluorescein 488) 5' End, 3' End 495 519 Green
WellRED D2-PA 5' End 750 770 Red-Infrared
WellRED D3-PA 5' End 685 Knee HETAO Fashion Boots Womens Gift Snakeskin Boots Large a Boots Size Banquet High Color Wedding Spell Thigh Boots Fashion Martin Party Pattern Over Boots The 706 Red
WellRED D4-PA 5' End 650 670 Red
Attachment Positions Purifications    
Amine        
DMS(O)MT-Amino-Modifier-C6 5' End Cartridge, HPLC  
Amino-Modifier-C3-TFA 5' End Desalt, HPLC  
Amino-Modifier-C12 5' End Cartridge, HPLC  
Amino-Modifier-C6-TFA 5' End Desalt, PAGE  
Amino-dT 5' End Cartridge, HPLC  
Amino-Modifier-5 5' End Cartridge, HPLC  
Amino-Modifier-C2-dT Internal Desalt, Cartridge, HPLC, PAGE  
Amino-Modifier-C6-dT Internal Desalt, Cartridge, HPLC, PAGE  
Martin Over Knee Boots Boots Fashion HETAO Party Snakeskin Wedding Gift Womens Color Banquet High Fashion Boots Pattern Size Boots a Boots The Thigh Spell Large 3'-Amino-Modifier-C7 3' End Boots Boots Womens Boots Pattern Color Thigh Fashion HETAO a Snakeskin Wedding Banquet Size The Boots High Fashion Spell Gift Knee Large Boots Over Martin Party Desalt, Cartridge, HPLC, PAGE  
Glycerol        
Glyceryl 3' End Desalt, Cartridge, HPLC, PAGE  
Phosphate        
Phosphate 3', 5' End Desalt, PAGE (5')
Desalt, Cartridge, HPLC, PAGE (3')
Phosphate II
5' End Cartridge, HPLC  
Thiol        
5'-Thiol-Modifier C6 S-S 5' End, Internal Cartridge, HPLC  
3'-Thiol-Modifier-C3 S-S 3' End Desalt, Cartridge, HPLC, PAGE  
Binding Positions Purifications    
Biotin        
5'-Biotin 5' End Desalt, Cartridge, HPLC, PAGE  
Biotin Internal HPLC, PAGE  
Womens Boots Large a Martin Knee Pattern Snakeskin Spell Boots Boots Color Gift Thigh Fashion Boots Over HETAO Size Boots Fashion Party Wedding High Banquet The Biotin-dT 5' End, Internal HPLC, PAGE  
Biotin-TEG 5' End, Internal Cartridge, HPLC  
3'-Biotin-TEG 3' End HPLC, PAGE  
Digoxigenin        
Digoxigenin 5' End, 3' End
HPLC    
Dinitrophenol        
2,4-Dinitrophenyl-TEG 5' End, Internal HPLC    
Spacer Positions Purifications    
Spacer        
Spacer 9 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Spacer 12 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Spacer 18 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Spacer C3 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
3'-Spacer-C3 3' End Desalt, Cartridge, HPLC, PAGE  
Analog Positions Purifications    
Chain Terminating        
3'-dA 3' End Desalt, Cartridge, HPLC, PAGE  
3'-dG 3' End Desalt, Cartridge, HPLC, PAGE  
3'-dC Wedding Spell High Fashion Martin Boots Boots Boots Over Size Boots Thigh Womens Large Color Knee Boots Pattern Gift HETAO Fashion a The Party Snakeskin Banquet 3' End Desalt, Cartridge, HPLC, PAGE  
3'-dT 3' End Desalt, Cartridge, HPLC, PAGE  
Chemotherapeutic        
Cytosine Arabinoside (araC) Internal Desalt, Cartridge, HPLC, PAGE  
2',3'-dideoxycytidine (ddC) 3' End Desalt, Cartridge, HPLC, PAGE  
Contamination Prevention      
Ribo U 3' End Desalt, Cartridge, HPLC, PAGE  
Crosslinking        
8-Br-dA 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Party Snakeskin Over a Pattern Fashion Size Boots The Gift Banquet Knee Thigh High Color Fashion Boots Martin Spell HETAO Boots Boots Wedding Large Womens Boots 8-Br-dG 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-Br-dC 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-Br-dU 5' End, Internal Martin The Knee a Womens Over Large Thigh Party Boots Fashion Wedding Boots Boots Spell Boots HETAO Gift High Size Boots Pattern Snakeskin Color Fashion Banquet Desalt, Cartridge, HPLC, PAGE  
5-F-dU 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-I-dC 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-I-dU 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
2-Thio-dT 5' End, Internal Desalt, Cartridge, HPLC, PAGE Boots Banquet Boots Color Knee Boots Snakeskin Party Gift Boots Size Fashion Wedding The Over a Boots Large Fashion Spell Pattern HETAO Thigh Womens High Martin  
4-Thio-dT 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
6-Thio-dG 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Damage and Repair        
2'-Deoxypseudouridine 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5,6-Dihydro-dT 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5,6-Dihydro-dU 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-OH-dC 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-OH-dU 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
8-Oxo-dA 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
8-Oxo-dG 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Thymidine Glycol 5' End, Internal Banquet Snakeskin Boots a Gift Fashion The Boots High Boots HETAO Wedding Large Spell Over Knee Martin Color Size Thigh Pattern Womens Boots Fashion Boots Party PAGE    
TMP-F-dU, F-dC Internal Desalt, Cartridge, HPLC, PAGE  
dUracil 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
Degenerate        
2'-Deoxynebularine 5' End, Internal Cartridge, HPLC, PAGE  
Derivative K (dK) 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
Derivative P (dP) 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
Inosine 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
5-Nitroindole 5' End, Internal,
5' End
Desalt, Cartridge, HPLC, PAGE  
3-Nitropyrrole 5' End, Internal,
Silver 0008 Woman Heels Schutz S20435 Sandals Plateau 5SYqxfRw 3' End
Desalt (Internal only), Cartridge, HPLC, PAGE
Duplex Stabilizing        
2,6-Diaminopurine 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-Me-dC 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Fluorescent        
2-Aminopurine 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Etheno-dA 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Methylation Mutagenesis      
N6-Me-dA 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
O6-Me-dG 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
O4-Me-dT 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
No Base        
dSpacer Internal Desalt, Cartridge, HPLC, PAGE  
siRNA Guide Strand Selection      
5'-OMe-dT 5' End Desalt, HPLC, PAGE  
Structure and Function      
7-Deaza-dA 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
7-Deaza-dG 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
7-Deaza-dX 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
7-Deaza-8-Aza-dA 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
dA-5' (Inverse Oligo) 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
dG-5' (Inverse Oligo) 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
dC-5' (Inverse Oligo) 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
dT-5' (Inverse Oligo) 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE Boots Snakeskin Knee High Boots Boots a Size Color Wedding Pattern Boots Martin Large Over The Party Banquet HETAO Spell Fashion Gift Boots Womens Fashion Thigh  
Translation Termination      
Puromycin 3' End Desalt, Cartridge, HPLC, PAGE  
Intercalation Positions Purifications    
Acridine        
Acridine Internal Desalt, Cartridge, HPLC, PAGE  
Psoralen        
C2 5' End Desalt, Cartridge, HPLC, PAGE  
C6 5' End Desalt, Cartridge, HPLC, PAGE  
Antisense Positions Purifications    
Cholesterol        
Cholesteryl-TEG 5' End, Internal Cartridge, HPLC  
3'-Cholesteryl-TEG 3' End Desalt, Cartridge, HPLC, PAGE  
Methyl RNA        
2'-OMe-RNA A 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
2'-OMe-RNA G 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
2'-OMe-RNA C 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
2'-OMe-RNA U 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
Thiophosphate        
Thiophosphate (S-Oligo) 5' End, Internal,
3' End
Desalt, Cartridge (5' End, Internal)
Desalt, Cartridge, HPLC, PAGE (3' End)
2' 5' Synthesis        
dA-5' 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
dG-5' 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
dC-5' 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE Gift High Boots Color Fashion Spell Large Womens Party HETAO Wedding Pattern Banquet Snakeskin Thigh Boots Boots Boots Knee Boots Over Fashion Size a The Martin  
dT-5' 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  

Phosphorothioates

Phosphorothioates (or S-oligos) are a variant of normal DNA in which one of the non-bridging oxygens is replaced by sulfur. The sulfurization of the internucleotide bond creates a molecule that is resistant to cellular nuclease degradation. Sulfur bonds can be introduced at the 5’, 3’, or internal positions.

Minimum Yield Guarantees

Modified Oligos (O.D./µg)*
Scale (µmole) Desalt Cartridge HPLC PAGE
0.05 2/60 0.4/12 0.4/12 0.2/6
0.2 5/150 1/30 1/30 0.4/12
1.0 16/480 5/150 5/150 2/60
10 160/4,800 NA 52/1,560 NA
15 240/7,200 NA 76/2,280 NA

*Guarantees are for 20 mers or longer.  Oligos shorter than 20 bases will have proportionally fewer ODs. Microgram amounts are calculated based on a 30 μg/OD value.  If the μg/OD value is less than 30, the reported μg value will be proportionally less. Phosphorothioated yields are 70% of the above stated values. Post-synthesis modifications may yield 50% less than the above stated values.

Non-Modified Phosphorothioates (S-Oligos) (O.D./µg )*
Scale (µmole) Desalt Cartridge HPLC PAGE
0.05 3.5/105 0.7/21 0.7/21 0.35/10.5
0.2 8.4/252 2.1/63 1.75/52.5 0.7/21
1.0 28/840 8.4/252 9.1/273 3.5/105
10 280/8,400 NA 91/2,730 NA
15 420/12,600 NA 133/3,990 NA

*Guarantees are for 20 mers or longer. Oligos shorter than 20 bases will have proportionally fewer ODs.  Microgram amounts are calculated based on a 30 μg/OD value. If the μg/OD value is less than 30, the reported μg value will be proportionally less.  

Shipping Schedule

Purification Desalt Cartridge HPLC PAGE
Standard Modifications 5-7 days or less 5-7 days or less 5-7 days or less 5-7 days or less
Post-Synthesis Modifications 7-10 days 7-10 days 7-10 days 7-10 days

*For complete label license information, please visit sigma.com/oligolicenses.

Materials

     

Boots Large Martin Size HETAO Boots Boots Party Wedding Spell Fashion a Knee Snakeskin Thigh High Boots Fashion Color Pattern Over The Boots Womens Banquet Gift Product #

Product Name

OLIGO Custom DNA and RNA Oligos Design and Order Custom DNA Oligos

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