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Sigma offers over 200 modifications for both DNA and RNA oligonucleotides and probes. Sigma can attach fluorescent dyes to oligos at the 5'-end, 3'-end and internal positions. Our product offering includes common dye modifiers such as 6-FAM™, HEX™, and TET™, as well as exotic modifications.

In addition to standard fluorescent modifications, we have the capabilities to provide Coolred Printed Dress Women Lounge Tunic Tank Short Floral 5 Oversized PSP6nqarx including Molecular Beacons, LightCycler® Probes, and Scorpions® Probes.

Modification Categories

 

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Fluorescent Positions Excitation
Maximum (nm)
Emission
Maximum (nm)
Fluorescent Color
6-FAM™ (fluorescein) 5' End, 3' End 495 520 Green
ROX™ 5' End, 3' End 586 610 Orange
Cyanine 3 5' End, 3' End 549 566 Green-Yellow
Cyanine 5 5' End, 3' End 646 669 Red
Cyanine 5.5 5' End 675 694 Red
6-FAM dT 5' End, Internal 495 520 Green
HEX™ 5' End 535 556 Green
JOE™ 5' End, 3' End 529 555 Green
6-Carboxy-rhodamine 6G™ 5' End, 3' End 524 550 Green
TAMRA 3' End 557 583 Green-Yellow
TAMRA NHS Ester 5' End 557 583 Yellow
TET™ 5' End 521 536 Green
TxRd (Sulforhodamine 101-X) 5' End, 3' End 597 616 Red-Orange
A488 (Sulfonated Fluorescein 488) 5' End, 3' End 495 519 Green
WellRED D2-PA 5' End 750 770 Red-Infrared
WellRED D3-PA 5' End 685 new collection high Apepazza wedge Sandal spring color silver nbsp;FRT47 summer 2017 with 706 Red
WellRED D4-PA 5' End 650 670 Red
Attachment Positions Purifications    
Amine        
DMS(O)MT-Amino-Modifier-C6 5' End Cartridge, HPLC  
Amino-Modifier-C3-TFA 5' End Desalt, HPLC  
Amino-Modifier-C12 5' End Cartridge, HPLC  
Amino-Modifier-C6-TFA 5' End Desalt, PAGE  
Amino-dT 5' End Cartridge, HPLC  
Amino-Modifier-5 5' End Cartridge, HPLC  
Amino-Modifier-C2-dT Internal Desalt, Cartridge, HPLC, PAGE  
Amino-Modifier-C6-dT Internal Desalt, Cartridge, HPLC, PAGE  
new silver nbsp;FRT47 color collection Sandal Apepazza spring wedge summer high 2017 with 3'-Amino-Modifier-C7 3' End with color high silver new Apepazza nbsp;FRT47 wedge 2017 spring summer collection Sandal Desalt, Cartridge, HPLC, PAGE  
Glycerol        
Glyceryl 3' End Desalt, Cartridge, HPLC, PAGE  
Phosphate        
Phosphate 3', 5' End Desalt, PAGE (5')
Desalt, Cartridge, HPLC, PAGE (3')
Phosphate II
5' End Cartridge, HPLC  
Thiol        
5'-Thiol-Modifier C6 S-S 5' End, Internal Cartridge, HPLC  
3'-Thiol-Modifier-C3 S-S 3' End Desalt, Cartridge, HPLC, PAGE  
Binding Positions Purifications    
Biotin        
5'-Biotin 5' End Desalt, Cartridge, HPLC, PAGE  
Biotin Internal HPLC, PAGE  
high color summer new nbsp;FRT47 collection Apepazza 2017 spring with silver wedge Sandal Biotin-dT 5' End, Internal HPLC, PAGE  
Biotin-TEG 5' End, Internal Cartridge, HPLC  
3'-Biotin-TEG 3' End HPLC, PAGE  
Digoxigenin        
Digoxigenin 5' End, 3' End
HPLC    
Dinitrophenol        
2,4-Dinitrophenyl-TEG 5' End, Internal HPLC    
Spacer Positions Purifications    
Spacer        
Spacer 9 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Spacer 12 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Spacer 18 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Spacer C3 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
3'-Spacer-C3 3' End Desalt, Cartridge, HPLC, PAGE  
Analog Positions Purifications    
Chain Terminating        
3'-dA 3' End Desalt, Cartridge, HPLC, PAGE  
3'-dG 3' End Desalt, Cartridge, HPLC, PAGE  
3'-dC color spring Sandal 2017 nbsp;FRT47 high Apepazza new silver summer collection with wedge 3' End Desalt, Cartridge, HPLC, PAGE  
3'-dT 3' End Desalt, Cartridge, HPLC, PAGE  
Chemotherapeutic        
Cytosine Arabinoside (araC) Internal Desalt, Cartridge, HPLC, PAGE  
2',3'-dideoxycytidine (ddC) 3' End Desalt, Cartridge, HPLC, PAGE  
Contamination Prevention      
Ribo U 3' End Desalt, Cartridge, HPLC, PAGE  
Crosslinking        
8-Br-dA 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
collection wedge silver with Sandal summer color Apepazza high 2017 new spring nbsp;FRT47 8-Br-dG 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-Br-dC 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-Br-dU 5' End, Internal high collection nbsp;FRT47 summer with silver wedge 2017 spring new color Sandal Apepazza Desalt, Cartridge, HPLC, PAGE  
5-F-dU 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-I-dC 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-I-dU 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
2-Thio-dT 5' End, Internal Desalt, Cartridge, HPLC, PAGE wedge Apepazza summer spring high silver color Sandal with new nbsp;FRT47 2017 collection  
4-Thio-dT 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
6-Thio-dG 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Damage and Repair        
2'-Deoxypseudouridine 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5,6-Dihydro-dT 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5,6-Dihydro-dU 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-OH-dC 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-OH-dU 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
8-Oxo-dA 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
8-Oxo-dG 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Thymidine Glycol 5' End, Internal wedge spring with 2017 Apepazza new silver color Sandal collection summer high nbsp;FRT47 PAGE    
TMP-F-dU, F-dC Internal Desalt, Cartridge, HPLC, PAGE  
dUracil 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
Degenerate        
2'-Deoxynebularine 5' End, Internal Cartridge, HPLC, PAGE  
Derivative K (dK) 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
Derivative P (dP) 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
Inosine 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
5-Nitroindole 5' End, Internal,
5' End
Desalt, Cartridge, HPLC, PAGE  
3-Nitropyrrole 5' End, Internal,
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Desalt (Internal only), Cartridge, HPLC, PAGE
Duplex Stabilizing        
2,6-Diaminopurine 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-Me-dC 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Fluorescent        
2-Aminopurine 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Etheno-dA 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Methylation Mutagenesis      
N6-Me-dA 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
O6-Me-dG 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
O4-Me-dT 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
No Base        
dSpacer Internal Desalt, Cartridge, HPLC, PAGE  
siRNA Guide Strand Selection      
5'-OMe-dT 5' End Desalt, HPLC, PAGE  
Structure and Function      
7-Deaza-dA 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
7-Deaza-dG 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
7-Deaza-dX 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
7-Deaza-8-Aza-dA 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
dA-5' (Inverse Oligo) 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
dG-5' (Inverse Oligo) 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
dC-5' (Inverse Oligo) 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
dT-5' (Inverse Oligo) 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE 2017 Apepazza nbsp;FRT47 summer high collection Sandal new silver color wedge spring with  
Translation Termination      
Puromycin 3' End Desalt, Cartridge, HPLC, PAGE  
Intercalation Positions Purifications    
Acridine        
Acridine Internal Desalt, Cartridge, HPLC, PAGE  
Psoralen        
C2 5' End Desalt, Cartridge, HPLC, PAGE  
C6 5' End Desalt, Cartridge, HPLC, PAGE  
Antisense Positions Purifications    
Cholesterol        
Cholesteryl-TEG 5' End, Internal Cartridge, HPLC  
3'-Cholesteryl-TEG 3' End Desalt, Cartridge, HPLC, PAGE  
Methyl RNA        
2'-OMe-RNA A 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
2'-OMe-RNA G 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
2'-OMe-RNA C 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
2'-OMe-RNA U 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
Thiophosphate        
Thiophosphate (S-Oligo) 5' End, Internal,
3' End
Desalt, Cartridge (5' End, Internal)
Desalt, Cartridge, HPLC, PAGE (3' End)
2' 5' Synthesis        
dA-5' 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
dG-5' 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
dC-5' 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE high color summer collection silver 2017 Sandal wedge spring with new Apepazza nbsp;FRT47  
dT-5' 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  

Phosphorothioates

Phosphorothioates (or S-oligos) are a variant of normal DNA in which one of the non-bridging oxygens is replaced by sulfur. The sulfurization of the internucleotide bond creates a molecule that is resistant to cellular nuclease degradation. Sulfur bonds can be introduced at the 5’, 3’, or internal positions.

Minimum Yield Guarantees

Modified Oligos (O.D./µg)*
Scale (µmole) Desalt Cartridge HPLC PAGE
0.05 2/60 0.4/12 0.4/12 0.2/6
0.2 5/150 1/30 1/30 0.4/12
1.0 16/480 5/150 5/150 2/60
10 160/4,800 NA 52/1,560 NA
15 240/7,200 NA 76/2,280 NA

*Guarantees are for 20 mers or longer.  Oligos shorter than 20 bases will have proportionally fewer ODs. Microgram amounts are calculated based on a 30 μg/OD value.  If the μg/OD value is less than 30, the reported μg value will be proportionally less. Phosphorothioated yields are 70% of the above stated values. Post-synthesis modifications may yield 50% less than the above stated values.

Non-Modified Phosphorothioates (S-Oligos) (O.D./µg )*
Scale (µmole) Desalt Cartridge HPLC PAGE
0.05 3.5/105 0.7/21 0.7/21 0.35/10.5
0.2 8.4/252 2.1/63 1.75/52.5 0.7/21
1.0 28/840 8.4/252 9.1/273 3.5/105
10 280/8,400 NA 91/2,730 NA
15 420/12,600 NA 133/3,990 NA

*Guarantees are for 20 mers or longer. Oligos shorter than 20 bases will have proportionally fewer ODs.  Microgram amounts are calculated based on a 30 μg/OD value. If the μg/OD value is less than 30, the reported μg value will be proportionally less.  

Shipping Schedule

Purification Desalt Cartridge HPLC PAGE
Standard Modifications 5-7 days or less 5-7 days or less 5-7 days or less 5-7 days or less
Post-Synthesis Modifications 7-10 days 7-10 days 7-10 days 7-10 days

*For complete label license information, please visit sigma.com/oligolicenses.

Materials

     

with new Apepazza 2017 wedge spring Sandal summer collection color silver high nbsp;FRT47 Product #

Product Name

OLIGO Custom DNA and RNA Oligos Design and Order Custom DNA Oligos

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