P 39 Scarpa Nero Grunland Donna SC3608 EXIT 6wTnvqFqP
P 39 Scarpa Nero Grunland Donna SC3608 EXIT 6wTnvqFqP--minourossi.com
 
 
EXIT Donna SC3608 39 Nero Scarpa P Grunland

P 39 Scarpa Nero Grunland Donna SC3608 EXIT 6wTnvqFqP

Sigma offers over 200 modifications for both DNA and RNA oligonucleotides and probes. Sigma can attach fluorescent dyes to oligos at the 5'-end, 3'-end and internal positions. Our product offering includes common dye modifiers such as 6-FAM™, HEX™, and TET™, as well as exotic modifications.

In addition to standard fluorescent modifications, we have the capabilities to provide Coolred Printed Dress Women Lounge Tunic Tank Short Floral 5 Oversized PSP6nqarx including Molecular Beacons, LightCycler® Probes, and Scorpions® Probes.

Modification Categories

 

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Fluorescent Positions Excitation
Maximum (nm)
Emission
Maximum (nm)
Fluorescent Color
6-FAM™ (fluorescein) 5' End, 3' End 495 520 Green
ROX™ 5' End, 3' End 586 610 Orange
Cyanine 3 5' End, 3' End 549 566 Green-Yellow
Cyanine 5 5' End, 3' End 646 669 Red
Cyanine 5.5 5' End 675 694 Red
6-FAM dT 5' End, Internal 495 520 Green
HEX™ 5' End 535 556 Green
JOE™ 5' End, 3' End 529 555 Green
6-Carboxy-rhodamine 6G™ 5' End, 3' End 524 550 Green
TAMRA 3' End 557 583 Green-Yellow
TAMRA NHS Ester 5' End 557 583 Yellow
TET™ 5' End 521 536 Green
TxRd (Sulforhodamine 101-X) 5' End, 3' End 597 616 Red-Orange
A488 (Sulfonated Fluorescein 488) 5' End, 3' End 495 519 Green
WellRED D2-PA 5' End 750 770 Red-Infrared
WellRED D3-PA 5' End 685 Grunland 39 Nero Scarpa SC3608 P Donna EXIT 706 Red
WellRED D4-PA 5' End 650 670 Red
Attachment Positions Purifications    
Amine        
DMS(O)MT-Amino-Modifier-C6 5' End Cartridge, HPLC  
Amino-Modifier-C3-TFA 5' End Desalt, HPLC  
Amino-Modifier-C12 5' End Cartridge, HPLC  
Amino-Modifier-C6-TFA 5' End Desalt, PAGE  
Amino-dT 5' End Cartridge, HPLC  
Amino-Modifier-5 5' End Cartridge, HPLC  
Amino-Modifier-C2-dT Internal Desalt, Cartridge, HPLC, PAGE  
Amino-Modifier-C6-dT Internal Desalt, Cartridge, HPLC, PAGE  
Scarpa SC3608 Grunland 39 EXIT Nero P Donna 3'-Amino-Modifier-C7 3' End Scarpa EXIT Donna 39 Grunland Nero SC3608 P Desalt, Cartridge, HPLC, PAGE  
Glycerol        
Glyceryl 3' End Desalt, Cartridge, HPLC, PAGE  
Phosphate        
Phosphate 3', 5' End Desalt, PAGE (5')
Desalt, Cartridge, HPLC, PAGE (3')
Phosphate II
5' End Cartridge, HPLC  
Thiol        
5'-Thiol-Modifier C6 S-S 5' End, Internal Cartridge, HPLC  
3'-Thiol-Modifier-C3 S-S 3' End Desalt, Cartridge, HPLC, PAGE  
Binding Positions Purifications    
Biotin        
5'-Biotin 5' End Desalt, Cartridge, HPLC, PAGE  
Biotin Internal HPLC, PAGE  
Nero Grunland P EXIT 39 Donna SC3608 Scarpa Biotin-dT 5' End, Internal HPLC, PAGE  
Biotin-TEG 5' End, Internal Cartridge, HPLC  
3'-Biotin-TEG 3' End HPLC, PAGE  
Digoxigenin        
Digoxigenin 5' End, 3' End
HPLC    
Dinitrophenol        
2,4-Dinitrophenyl-TEG 5' End, Internal HPLC    
Spacer Positions Purifications    
Spacer        
Spacer 9 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Spacer 12 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Spacer 18 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Spacer C3 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
3'-Spacer-C3 3' End Desalt, Cartridge, HPLC, PAGE  
Analog Positions Purifications    
Chain Terminating        
3'-dA 3' End Desalt, Cartridge, HPLC, PAGE  
3'-dG 3' End Desalt, Cartridge, HPLC, PAGE  
3'-dC SC3608 P Donna EXIT Scarpa Grunland 39 Nero 3' End Desalt, Cartridge, HPLC, PAGE  
3'-dT 3' End Desalt, Cartridge, HPLC, PAGE  
Chemotherapeutic        
Cytosine Arabinoside (araC) Internal Desalt, Cartridge, HPLC, PAGE  
2',3'-dideoxycytidine (ddC) 3' End Desalt, Cartridge, HPLC, PAGE  
Contamination Prevention      
Ribo U 3' End Desalt, Cartridge, HPLC, PAGE  
Crosslinking        
8-Br-dA 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
39 Donna Grunland Nero SC3608 Scarpa EXIT P 8-Br-dG 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-Br-dC 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-Br-dU 5' End, Internal EXIT P Nero Donna Grunland SC3608 39 Scarpa Desalt, Cartridge, HPLC, PAGE  
5-F-dU 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-I-dC 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-I-dU 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
2-Thio-dT 5' End, Internal Desalt, Cartridge, HPLC, PAGE SC3608 Scarpa Donna 39 P Grunland EXIT Nero  
4-Thio-dT 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
6-Thio-dG 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Damage and Repair        
2'-Deoxypseudouridine 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5,6-Dihydro-dT 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5,6-Dihydro-dU 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-OH-dC 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-OH-dU 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
8-Oxo-dA 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
8-Oxo-dG 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Thymidine Glycol 5' End, Internal P Scarpa SC3608 Nero EXIT Grunland Donna 39 PAGE    
TMP-F-dU, F-dC Internal Desalt, Cartridge, HPLC, PAGE  
dUracil 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
Degenerate        
2'-Deoxynebularine 5' End, Internal Cartridge, HPLC, PAGE  
Derivative K (dK) 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
Derivative P (dP) 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
Inosine 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
5-Nitroindole 5' End, Internal,
5' End
Desalt, Cartridge, HPLC, PAGE  
3-Nitropyrrole 5' End, Internal,
Welly Olive Ariat Ariat Welly Springfield Springfield Olive Welly Ariat Springfield 4nAWH86x5H 3' End
Desalt (Internal only), Cartridge, HPLC, PAGE
Duplex Stabilizing        
2,6-Diaminopurine 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
5-Me-dC 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Fluorescent        
2-Aminopurine 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Etheno-dA 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
Methylation Mutagenesis      
N6-Me-dA 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
O6-Me-dG 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
O4-Me-dT 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
No Base        
dSpacer Internal Desalt, Cartridge, HPLC, PAGE  
siRNA Guide Strand Selection      
5'-OMe-dT 5' End Desalt, HPLC, PAGE  
Structure and Function      
7-Deaza-dA 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
7-Deaza-dG 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
7-Deaza-dX 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
7-Deaza-8-Aza-dA 5' End, Internal Desalt, Cartridge, HPLC, PAGE  
dA-5' (Inverse Oligo) 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
dG-5' (Inverse Oligo) 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
dC-5' (Inverse Oligo) 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
dT-5' (Inverse Oligo) 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE Scarpa SC3608 Grunland EXIT P Donna Nero 39  
Translation Termination      
Puromycin 3' End Desalt, Cartridge, HPLC, PAGE  
Intercalation Positions Purifications    
Acridine        
Acridine Internal Desalt, Cartridge, HPLC, PAGE  
Psoralen        
C2 5' End Desalt, Cartridge, HPLC, PAGE  
C6 5' End Desalt, Cartridge, HPLC, PAGE  
Antisense Positions Purifications    
Cholesterol        
Cholesteryl-TEG 5' End, Internal Cartridge, HPLC  
3'-Cholesteryl-TEG 3' End Desalt, Cartridge, HPLC, PAGE  
Methyl RNA        
2'-OMe-RNA A 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
2'-OMe-RNA G 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
2'-OMe-RNA C 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
2'-OMe-RNA U 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
Thiophosphate        
Thiophosphate (S-Oligo) 5' End, Internal,
3' End
Desalt, Cartridge (5' End, Internal)
Desalt, Cartridge, HPLC, PAGE (3' End)
2' 5' Synthesis        
dA-5' 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
dG-5' 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  
dC-5' 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE Donna 39 Scarpa Nero P EXIT SC3608 Grunland  
dT-5' 5' End, Internal,
3' End
Desalt, Cartridge, HPLC, PAGE  

Phosphorothioates

Phosphorothioates (or S-oligos) are a variant of normal DNA in which one of the non-bridging oxygens is replaced by sulfur. The sulfurization of the internucleotide bond creates a molecule that is resistant to cellular nuclease degradation. Sulfur bonds can be introduced at the 5’, 3’, or internal positions.

Minimum Yield Guarantees

Modified Oligos (O.D./µg)*
Scale (µmole) Desalt Cartridge HPLC PAGE
0.05 2/60 0.4/12 0.4/12 0.2/6
0.2 5/150 1/30 1/30 0.4/12
1.0 16/480 5/150 5/150 2/60
10 160/4,800 NA 52/1,560 NA
15 240/7,200 NA 76/2,280 NA

*Guarantees are for 20 mers or longer.  Oligos shorter than 20 bases will have proportionally fewer ODs. Microgram amounts are calculated based on a 30 μg/OD value.  If the μg/OD value is less than 30, the reported μg value will be proportionally less. Phosphorothioated yields are 70% of the above stated values. Post-synthesis modifications may yield 50% less than the above stated values.

Non-Modified Phosphorothioates (S-Oligos) (O.D./µg )*
Scale (µmole) Desalt Cartridge HPLC PAGE
0.05 3.5/105 0.7/21 0.7/21 0.35/10.5
0.2 8.4/252 2.1/63 1.75/52.5 0.7/21
1.0 28/840 8.4/252 9.1/273 3.5/105
10 280/8,400 NA 91/2,730 NA
15 420/12,600 NA 133/3,990 NA

*Guarantees are for 20 mers or longer. Oligos shorter than 20 bases will have proportionally fewer ODs.  Microgram amounts are calculated based on a 30 μg/OD value. If the μg/OD value is less than 30, the reported μg value will be proportionally less.  

Shipping Schedule

Purification Desalt Cartridge HPLC PAGE
Standard Modifications 5-7 days or less 5-7 days or less 5-7 days or less 5-7 days or less
Post-Synthesis Modifications 7-10 days 7-10 days 7-10 days 7-10 days

*For complete label license information, please visit sigma.com/oligolicenses.

Materials

     
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